Reporter

Part:BBa_K2271021:Design

Designed by: Remy Bastiaan Tjeng   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


pHlourin2 - pH sensitive green flourescent protein for yeast expression

pHlourin2 is a ratiometric flourescent reporter protein with a bimodal excitation spectrum with peaks at 395nm and 475nm at emission of 509nm. Physiological range tested is between 5.8 and 7.8 pH with a foldchange of 3.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


Design Notes

Yeast codon optimization. No start and stop codon.


Source

Synthese from IDT. Protein sequence obtained from (Mahon M. J. et al., 2011)

References

(Mahon M. J. et al., 2011)